{"id":3792,"date":"2017-12-11T04:06:26","date_gmt":"2017-12-11T04:06:26","guid":{"rendered":"https:\/\/www.enago.tw\/blog\/?p=3792"},"modified":"2020-07-25T15:17:29","modified_gmt":"2020-07-25T15:17:29","slug":"seek-blastn%ef%bc%9a%e5%9c%a8%e7%99%8c%e7%97%87%e7%a0%94%e7%a9%b6%e8%ab%96%e6%96%87%e4%b8%ad%e6%aa%a2%e6%b8%ac%e5%87%ba%e5%9f%ba%e5%9b%a0%e5%ba%8f%e5%88%97%e4%b8%ad%e7%9a%84%e9%8c%af%e8%aa%a4","status":"publish","type":"post","link":"https:\/\/www.ulatus.tw\/academy\/seek-blastn%ef%bc%9a%e5%9c%a8%e7%99%8c%e7%97%87%e7%a0%94%e7%a9%b6%e8%ab%96%e6%96%87%e4%b8%ad%e6%aa%a2%e6%b8%ac%e5%87%ba%e5%9f%ba%e5%9b%a0%e5%ba%8f%e5%88%97%e4%b8%ad%e7%9a%84%e9%8c%af%e8%aa%a4\/","title":{"rendered":"Seek &#038; Blastn\uff1a\u5728\u764c\u75c7\u7814\u7a76\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u5e8f\u5217\u4e2d\u7684\u932f\u8aa4"},"content":{"rendered":"<p>2017\u5e7410\u6708\u521d\uff0c\u79d1\u5b78\u5bb6Jennifer Byrne\u548cCyril Labb\u00e9\u5206\u4eab\u4e86\u4e00\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u53ef\u4ee5\u5728\u5df2\u7d93\u767c\u8868\u7684\u7814\u7a76\u5be6\u9a57\u4e2d\uff0c\u6aa2\u6e2c\u51fa\u4e0d\u6b63\u78ba\u7684\u57fa\u56e0\u5e8f\u5217\u3002\u5229\u7528\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u7684\u8ad6\u6587\u4e2d\uff0c\u767c\u73fe\u8d85\u904e\u4e8660\u7bc7\u6709\u5be6\u9a57\u7f3a\u9677\u3002\u9019\u5169\u4f4d\u79d1\u5b78\u5bb6\u662f\u5c07\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u548c\u96fb\u8166\u7684\u5c08\u696d\u77e5\u8b58\u7d50\u5408\u8d77\u4f86\uff0c\u958b\u767c\u548c\u5c0e\u5165\u8edf\u9ad4\u300cSeek &amp; Blastn\u300d\u3002\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\u73fe\u5728\u6b63\u8655\u65bc\u6e2c\u8a66\u968e\u6bb5\uff0c\u53ef\u4f9b\u540c\u5115\u7814\u7a76\u4eba\u54e1\u4f5c\u7dda\u4e0a\u6e2c\u8a66\u548c\u6539\u9032\u3002\u4e0b\u4e00\u6b65\u5c07\u9032\u884c\u5546\u696d\u5316\uff0c\u4e26\u5411\u51fa\u7248\u5546\u548c\u671f\u520a\u7de8\u8f2f\u63a8\u85a6\u8a72\u8edf\u9ad4\u3002<\/p>\n<p>\u81ea2015\u5e74\u4ee5\u4f86\uff0cByrne\u4fbf\u81f4\u529b\u65bc\u8b58\u5225\u4eba\u985e\u764c\u75c7\u8ad6\u6587\u4e2d\u7684\u932f\u8aa4\uff0c\u57285\u7bc7\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u529f\u80fd\u7684\u932f\u8aa4\u3002\u5728\u5be9\u67e5\u904e\u7a0b\u4e2d\uff0c\u767c\u73fe\u5831\u544a\u4e2d\u7684\u6838\u82f7\u9178\u5e8f\u5217(nucleotide sequences)\u662f\u4e0d\u6b63\u78ba\u7684\uff0c\u56e0\u6b64\u6709\u5169\u7bc7\u8ad6\u6587\u906d\u5230\u64a4\u56de\u3002\u5f8c\u7e8c\u9084\u6709\u5169\u7bc7\u8ad6\u6587\u572811\u670821\u65e5\u4ea6\u906d\u64a4\u56de\u3002\u9019\u4e9b\u932f\u8aa4\u53c8\u51fa\u73fe\u5728\u5176\u4ed625\u7bc7\u8ad6\u6587\u4e2d\uff0c\u4e5f\u4fc3\u6210Byrne\u548cLabb\u00e9\u6b63\u5f0f\u958b\u767c\u548c\u5c0e\u5165\u300cSeek &amp; Blastn\u300d\u6aa2\u6e2c\u5de5\u5177\u3002<\/p>\n<p>\u5c0d\u65bc\u9019\u5957\u6aa2\u6e2c\u5de5\u5177\u7684\u539f\u7406\uff0c\u7c21\u800c\u8a00\u4e4b\uff0c\u5f9e\u4e0a\u50b3\u5230\u8edf\u9ad4\u7684\u4efb\u4f55\u7d66\u5b9a\u8ad6\u6587\u4e2d\u6240\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9032\u884c\u57fa\u65bc\u4e8b\u5be6\u7684\u6e2c\u8a66\u3002\u5f9e\u6280\u8853\u4f86\u8aaa\uff0c\u8edf\u9ad4\u7a0b\u5f0f\u4ea4\u53c9\u6bd4\u5c0d\u6aa2\u67e5\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9019\u662f\u7a31\u70ba\u6838\u82f7\u9178\u57fa\u672c\u5c40\u90e8\u8abf\u6574\u641c\u7d22\u5de5\u5177\uff08Nucleotide Basic Local Alignment Search tool\uff0cBLAST\uff09\u7684\u516c\u5171\u8cc7\u6599\u5eab\u3002\u5982\u679c\u63cf\u8ff0\u7684\u76ee\u6a19\u4eba\u985e\u57fa\u56e0\u5e8f\u5217\u8207Blastn\u8cc7\u6599\u5eab\u4e0d\u5339\u914d\uff0c\u5247\u6703\u6a19\u8a18\u932f\u8aa4\u3002\u76f8\u53cd\u7684\uff0c\u534a\u81ea\u52d5\u5316\u8edf\u9ad4\u7a0b\u5f0f\u4e5f\u53ef\u4ee5\u6aa2\u6e2c\u88ab\u63cf\u8ff0\u70ba\u975e\u76ee\u6a19\u7684\u5e8f\u5217\uff0c\u4e26\u4e14\u8207Blastn\u8cc7\u6599\u5eab\u5339\u914d\u3002\u5118\u7ba1\u76ee\u524d\u50c5\u9650\u65bc\u4eba\u985e\u5e8f\u5217\uff0c\u4f46\u662f\u5b83\u5011\u9084\u5e0c\u671b\u5305\u62ec\u5176\u4ed6\u7269\u7a2e\u7684\u5e8f\u5217\u9a57\u8b49\u3002<\/p>\n<p>\u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u57289\u6708\u4efd\u5411\u570b\u969b\u540c\u5115\u5be9\u67e5\u548c\u79d1\u5b78\u51fa\u7248\u6703\u8b70\u63d0\u4ea4\u4e86\u4ed6\u5011\u8a08\u5283\u7684\u65e9\u671f\u7814\u7a76\u6210\u679c\u3002\u5728\u6703\u8b70\u8a18\u9304\u4e2d\u6982\u8ff0\u4e86\u8a72\u8a08\u5283\u5982\u4f55\u63d0\u9ad8\u79d1\u5b78\u54c1\u8cea\u548c\u4fe1\u8b7d\u3002\u6839\u64da\u8a2d\u8a08\uff0c\u300cSeek &amp; Blastn\u300d\u534a\u81ea\u52d5\u5316\u5de5\u5177\u4f7f\u7528\u5be6\u9ad4\u8b58\u5225\u6280\u8853\u63d0\u53d6\u4e86\u57fa\u56e0\u6a19\u8b58\u7b26\u865f\uff08gene identifier\uff09\u548c\u6838\u82f7\u9178\u5e8f\u5217\uff08nucleotide sequences\uff09\u3002\u4f7f\u7528\u6a5f\u5668\u81ea\u52d5\u5206\u6790\u5305\u542b\u6bcf\u500b\u5e8f\u5217\u7684\u57fa\u56e0\u53e5\uff0c\u8207blastn\u5206\u6790\u76f8\u6bd4\u8f03\uff0c\u9078\u5b9a\u4e00\u500b\u8072\u7a31\u72c0\u614b\u3002Google Scholar\u53ef\u4ee5\u9032\u4e00\u6b65\u8a55\u4f30\u6587\u737b\u4e2d\u7684\u8072\u7a31\u72c0\u614b\u3002\u9996\u5148\uff0c\u7814\u7a76\u9451\u5225\u51fa\u4e00\u7d44\u9ad8\u5ea6\u76f8\u4f3c\u7684\u764c\u75c7\u7814\u7a76\u51fa\u7248\u7269\uff08CorpusP\uff09\u3002\u9019\u9805\u7814\u7a76\u9032\u4e00\u6b65\u5305\u62ec\u4e86\u53e6\u5916\u4e00\u7d44154\u500b\u672a\u77e5\u7814\u7a76\uff08CorpusU\uff09\u3002<\/p>\n<p>\u6839\u64da\u9019\u9805\u7814\u7a76\uff0c\u4f7f\u7528\u300cSeek &amp; Blastn\u300d\u5728\u9019\u4e9b\u51fa\u7248\u7269\u63d0\u53d6\u4e86\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u5176\u4e2dCorpusP\u670948\/48 (100%)\uff0c\u4ee5\u53caCorpusU\u6709111\/154(72%)\u3002\u5728CorpusP\u4e2d\u6a19\u8a18\u4e86\u4e0d\u6b63\u78ba\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u536038\/48 (79%)\u3002<\/p>\n<p>\u6b64\u5916\uff0c\u8a72\u5de5\u5177\u8868\u660e\uff0c\u8207\u76ee\u6a19\u5e8f\u5217(targeting sequences)\u76f8\u6bd4\uff0c\u975e\u76ee\u6a19\u5e8f\u5217(nontargeting sequences)\u7684\u9810\u6e2c\u662f\u4e0d\u6b63\u78ba\u7684\u3002\u6aa2\u51fa\u6838\u82f7\u9178\u5e8f\u5217\u4e0d\u6b63\u78ba\u536030\/154\uff0819%\uff09\uff0c\u96d6\u7136\u7570\u5e38\u6bd4\u4f8b\u5f88\u5c0f\uff0c\u4f46\u4e8b\u5be6\u67e5\u6838\u7a0b\u5f0f\u4ecd\u7136\u5b58\u5728\u5be6\u8cea\u6027\u7684\u932f\u8aa4\u3002\u56e0\u6b64\uff0c\u5c07\u8ad6\u6587\u4e1f\u9032\u8edf\u9ad4\u67e5\u6838\u5f8c\uff0c\u76ee\u524d\u9084\u9700\u8981\u984d\u5916\u7684\u4eba\u5de5\u67e5\u6838\u3002\u7576\u57fa\u56e0\u8fa8\u8b58\u767c\u751f\u8b8a\u7570\u6642\uff0c\u7a0b\u5f0f\u4e5f\u6703\u5728\u76ee\u6a19\u5e8f\u5217\u5206\u6790\u4e2d\u4e5f\u6709\u98a8\u96aa\u3002\u76ee\u524dSeek &amp; Blastn\u53ea\u662f\u4e00\u500b\u5c0f\u898f\u6a21\u6e2c\u8a66\u6027\u8cea\u7684\u7a0b\u5f0f\uff0c\u4ecd\u9700\u8981\u751f\u91ab\u5b78\u754c\u7684\u540c\u5115\u5be9\u67e5\u4f5c\u5f8c\u7e8c\u7684\u5b8c\u6574\u5206\u6790\u3002<\/p>\n<p>\u5118\u7ba1\u8a72\u8edf\u9ad4\u8655\u65bc\u521d\u6b65\u72c0\u614b\uff0c\u4f46\u5b83\u7a81\u986f\u51fa\u4e86\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u7684\u4e00\u4e9b\u6838\u5fc3\u554f\u984c\u3002\u4f8b\u5982\uff0c\u5728\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u9a57\u8b49\u51fa\u7684\u5e8f\u5217\u4e0d\u5339\u914d\uff0c\u53ef\u4ee5\u4f7f\u8ad6\u6587\u7684\u7d50\u679c\u548c\u7d50\u8ad6\u7121\u6548\u3002\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u9451\u5225\u7d50\u679c\u53ef\u8aaa\u660e\uff1a\u8ad6\u6587\u4e2d\u7684\u7d50\u679c\u7121\u6cd5\u5c0d\u539f\u59cb\u5be6\u9a57\u4f5c\u771f\u5be6\u7684\u53cd\u6620\u3002\u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u7e3d\u5171\u572890\u591a\u7bc7\u8ad6\u6587\u4e2d\u767c\u73fe\u4e86\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u3002\u5f9e\u5982\u6b64\u7684\u932f\u8aa4\u770b\u4f86\uff0c\u6b63\u5728\u9032\u884c\u4e2d\u7684\u7814\u7a76\uff0c\u5176\u6709\u6548\u6027\u548c\u65b9\u6cd5\u5c0d\u65bc\u672a\u4f86\u7684\u5be6\u9a57\u53ef\u8907\u88fd\u6027(reproducibility)\u6709\u8457\u56b4\u91cd\u7684\u5f71\u97ff\u3002<\/p>\n<p>\u5229\u7528\u300cSeek &amp; Blastn\u300d\u53ef\u4fc3\u9032\u826f\u597d\u7684\u79d1\u5b78\u5be6\u8e10\uff0c\u800c\u975e\u8d95\u76e1\u6bba\u7d55\u3002\u671f\u671b\u9019\u4e00\u985e\u5de5\u5177\u5c07\u53ef\u4f7f\u932f\u8aa4\u7387\u91cf\u5316\uff0c\u4ee5\u63a7\u7ba1\u9810\u9632\u8907\u88fd\u5be6\u9a57\u7684\u5371\u6a5f\u3002\u6b64\u5916\uff0cLabb\u00e9\u5148\u524d\u4f7f120\u7bc7\u8ad6\u6587\u906d\u64a4\u56de\uff0c\u9019\u4e5f\u662f\u4fc3\u6210\u51fa\u7248\u5546\u4f7f\u7528Seek &amp; Blastn\u5de5\u5177\u4f5c\u70ba\u6587\u7ae0\u7be9\u9078\u904e\u7a0b\u7684\u52d5\u529b\u3002\u57fa\u65bc\u8edf\u9ad4\u7684\u6210\u672c\u3001\u6e96\u78ba\u6027\u548c\u6613\u7528\u6027\uff0c\u5c07\u53ef\u671b\u63d0\u9ad8\u73fe\u6709\u7684\u5b78\u8853\u51fa\u7248\u6a19\u6e96\u3002\u5b78\u8853\u671f\u520a\u7684\u7de8\u8f2f\u53ef\u4ee5\u8207\u4e4b\u5171\u540c\u958b\u767c\u8edf\u9ad4\u7684\u8a66\u7528\u7248\u672c\uff0c\u5728\u7a69\u5b9a\u6210\u719f\u4e26\u4e14\u5efa\u7acb\u53ef\u4fe1\u5ea6\u4e4b\u5f8c\uff0c\u6709\u5229\u65bc\u96a8\u5f8c\u5c07\u9032\u884c\u7684\u5546\u696d\u5316\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>2017\u5e7410\u6708\u521d\uff0c\u79d1\u5b78\u5bb6Jennifer Byrne\u548cCyril Labb\u00e9\u5206\u4eab\u4e86\u4e00\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u53ef\u4ee5\u5728\u5df2\u7d93\u767c\u8868\u7684\u7814\u7a76\u5be6\u9a57\u4e2d\uff0c\u6aa2\u6e2c\u51fa\u4e0d\u6b63\u78ba\u7684\u57fa\u56e0\u5e8f\u5217\u3002\u5229\u7528\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u7684\u8ad6\u6587\u4e2d\uff0c\u767c\u73fe\u8d85\u904e\u4e8660\u7bc7\u6709\u5be6\u9a57\u7f3a\u9677\u3002\u9019\u5169\u4f4d\u79d1\u5b78\u5bb6\u662f\u5c07\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u548c\u96fb\u8166\u7684\u5c08\u696d\u77e5\u8b58\u7d50\u5408\u8d77\u4f86\uff0c\u958b\u767c\u548c\u5c0e\u5165\u8edf\u9ad4\u300cSeek &amp; Blastn\u300d\u3002\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\u73fe\u5728\u6b63\u8655\u65bc\u6e2c\u8a66\u968e\u6bb5\uff0c\u53ef\u4f9b\u540c\u5115\u7814\u7a76\u4eba\u54e1\u4f5c\u7dda\u4e0a\u6e2c\u8a66\u548c\u6539\u9032\u3002\u4e0b\u4e00\u6b65\u5c07\u9032\u884c\u5546\u696d\u5316\uff0c\u4e26\u5411\u51fa\u7248\u5546\u548c\u671f\u520a\u7de8\u8f2f\u63a8\u85a6\u8a72\u8edf\u9ad4\u3002 \u81ea2015\u5e74\u4ee5\u4f86\uff0cByrne\u4fbf\u81f4\u529b\u65bc\u8b58\u5225\u4eba\u985e\u764c\u75c7\u8ad6\u6587\u4e2d\u7684\u932f\u8aa4\uff0c\u57285\u7bc7\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u529f\u80fd\u7684\u932f\u8aa4\u3002\u5728\u5be9\u67e5\u904e\u7a0b\u4e2d\uff0c\u767c\u73fe\u5831\u544a\u4e2d\u7684\u6838\u82f7\u9178\u5e8f\u5217(nucleotide sequences)\u662f\u4e0d\u6b63\u78ba\u7684\uff0c\u56e0\u6b64\u6709\u5169\u7bc7\u8ad6\u6587\u906d\u5230\u64a4\u56de\u3002\u5f8c\u7e8c\u9084\u6709\u5169\u7bc7\u8ad6\u6587\u572811\u670821\u65e5\u4ea6\u906d\u64a4\u56de\u3002\u9019\u4e9b\u932f\u8aa4\u53c8\u51fa\u73fe\u5728\u5176\u4ed625\u7bc7\u8ad6\u6587\u4e2d\uff0c\u4e5f\u4fc3\u6210Byrne\u548cLabb\u00e9\u6b63\u5f0f\u958b\u767c\u548c\u5c0e\u5165\u300cSeek &amp; Blastn\u300d\u6aa2\u6e2c\u5de5\u5177\u3002 \u5c0d\u65bc\u9019\u5957\u6aa2\u6e2c\u5de5\u5177\u7684\u539f\u7406\uff0c\u7c21\u800c\u8a00\u4e4b\uff0c\u5f9e\u4e0a\u50b3\u5230\u8edf\u9ad4\u7684\u4efb\u4f55\u7d66\u5b9a\u8ad6\u6587\u4e2d\u6240\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9032\u884c\u57fa\u65bc\u4e8b\u5be6\u7684\u6e2c\u8a66\u3002\u5f9e\u6280\u8853\u4f86\u8aaa\uff0c\u8edf\u9ad4\u7a0b\u5f0f\u4ea4\u53c9\u6bd4\u5c0d\u6aa2\u67e5\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9019\u662f\u7a31\u70ba\u6838\u82f7\u9178\u57fa\u672c\u5c40\u90e8\u8abf\u6574\u641c\u7d22\u5de5\u5177\uff08Nucleotide Basic Local Alignment Search tool\uff0cBLAST\uff09\u7684\u516c\u5171\u8cc7\u6599\u5eab\u3002\u5982\u679c\u63cf\u8ff0\u7684\u76ee\u6a19\u4eba\u985e\u57fa\u56e0\u5e8f\u5217\u8207Blastn\u8cc7\u6599\u5eab\u4e0d\u5339\u914d\uff0c\u5247\u6703\u6a19\u8a18\u932f\u8aa4\u3002\u76f8\u53cd\u7684\uff0c\u534a\u81ea\u52d5\u5316\u8edf\u9ad4\u7a0b\u5f0f\u4e5f\u53ef\u4ee5\u6aa2\u6e2c\u88ab\u63cf\u8ff0\u70ba\u975e\u76ee\u6a19\u7684\u5e8f\u5217\uff0c\u4e26\u4e14\u8207Blastn\u8cc7\u6599\u5eab\u5339\u914d\u3002\u5118\u7ba1\u76ee\u524d\u50c5\u9650\u65bc\u4eba\u985e\u5e8f\u5217\uff0c\u4f46\u662f\u5b83\u5011\u9084\u5e0c\u671b\u5305\u62ec\u5176\u4ed6\u7269\u7a2e\u7684\u5e8f\u5217\u9a57\u8b49\u3002 \u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u57289\u6708\u4efd\u5411\u570b\u969b\u540c\u5115\u5be9\u67e5\u548c\u79d1\u5b78\u51fa\u7248\u6703\u8b70\u63d0\u4ea4\u4e86\u4ed6\u5011\u8a08\u5283\u7684\u65e9\u671f\u7814\u7a76\u6210\u679c\u3002\u5728\u6703\u8b70\u8a18\u9304\u4e2d\u6982\u8ff0\u4e86\u8a72\u8a08\u5283\u5982\u4f55\u63d0\u9ad8\u79d1\u5b78\u54c1\u8cea\u548c\u4fe1\u8b7d\u3002\u6839\u64da\u8a2d\u8a08\uff0c\u300cSeek &amp; Blastn\u300d\u534a\u81ea\u52d5\u5316\u5de5\u5177\u4f7f\u7528\u5be6\u9ad4\u8b58\u5225\u6280\u8853\u63d0\u53d6\u4e86\u57fa\u56e0\u6a19\u8b58\u7b26\u865f\uff08gene identifier\uff09\u548c\u6838\u82f7\u9178\u5e8f\u5217\uff08nucleotide sequences\uff09\u3002\u4f7f\u7528\u6a5f\u5668\u81ea\u52d5\u5206\u6790\u5305\u542b\u6bcf\u500b\u5e8f\u5217\u7684\u57fa\u56e0\u53e5\uff0c\u8207blastn\u5206\u6790\u76f8\u6bd4\u8f03\uff0c\u9078\u5b9a\u4e00\u500b\u8072\u7a31\u72c0\u614b\u3002Google Scholar\u53ef\u4ee5\u9032\u4e00\u6b65\u8a55\u4f30\u6587\u737b\u4e2d\u7684\u8072\u7a31\u72c0\u614b\u3002\u9996\u5148\uff0c\u7814\u7a76\u9451\u5225\u51fa\u4e00\u7d44\u9ad8\u5ea6\u76f8\u4f3c\u7684\u764c\u75c7\u7814\u7a76\u51fa\u7248\u7269\uff08CorpusP\uff09\u3002\u9019\u9805\u7814\u7a76\u9032\u4e00\u6b65\u5305\u62ec\u4e86\u53e6\u5916\u4e00\u7d44154\u500b\u672a\u77e5\u7814\u7a76\uff08CorpusU\uff09\u3002 \u6839\u64da\u9019\u9805\u7814\u7a76\uff0c\u4f7f\u7528\u300cSeek &amp; Blastn\u300d\u5728\u9019\u4e9b\u51fa\u7248\u7269\u63d0\u53d6\u4e86\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u5176\u4e2dCorpusP\u670948\/48 (100%)\uff0c\u4ee5\u53caCorpusU\u6709111\/154(72%)\u3002\u5728CorpusP\u4e2d\u6a19\u8a18\u4e86\u4e0d\u6b63\u78ba\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u536038\/48 (79%)\u3002 \u6b64\u5916\uff0c\u8a72\u5de5\u5177\u8868\u660e\uff0c\u8207\u76ee\u6a19\u5e8f\u5217(targeting sequences)\u76f8\u6bd4\uff0c\u975e\u76ee\u6a19\u5e8f\u5217(nontargeting sequences)\u7684\u9810\u6e2c\u662f\u4e0d\u6b63\u78ba\u7684\u3002\u6aa2\u51fa\u6838\u82f7\u9178\u5e8f\u5217\u4e0d\u6b63\u78ba\u536030\/154\uff0819%\uff09\uff0c\u96d6\u7136\u7570\u5e38\u6bd4\u4f8b\u5f88\u5c0f\uff0c\u4f46\u4e8b\u5be6\u67e5\u6838\u7a0b\u5f0f\u4ecd\u7136\u5b58\u5728\u5be6\u8cea\u6027\u7684\u932f\u8aa4\u3002\u56e0\u6b64\uff0c\u5c07\u8ad6\u6587\u4e1f\u9032\u8edf\u9ad4\u67e5\u6838\u5f8c\uff0c\u76ee\u524d\u9084\u9700\u8981\u984d\u5916\u7684\u4eba\u5de5\u67e5\u6838\u3002\u7576\u57fa\u56e0\u8fa8\u8b58\u767c\u751f\u8b8a\u7570\u6642\uff0c\u7a0b\u5f0f\u4e5f\u6703\u5728\u76ee\u6a19\u5e8f\u5217\u5206\u6790\u4e2d\u4e5f\u6709\u98a8\u96aa\u3002\u76ee\u524dSeek &amp; Blastn\u53ea\u662f\u4e00\u500b\u5c0f\u898f\u6a21\u6e2c\u8a66\u6027\u8cea\u7684\u7a0b\u5f0f\uff0c\u4ecd\u9700\u8981\u751f\u91ab\u5b78\u754c\u7684\u540c\u5115\u5be9\u67e5\u4f5c\u5f8c\u7e8c\u7684\u5b8c\u6574\u5206\u6790\u3002 \u5118\u7ba1\u8a72\u8edf\u9ad4\u8655\u65bc\u521d\u6b65\u72c0\u614b\uff0c\u4f46\u5b83\u7a81\u986f\u51fa\u4e86\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u7684\u4e00\u4e9b\u6838\u5fc3\u554f\u984c\u3002\u4f8b\u5982\uff0c\u5728\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u9a57\u8b49\u51fa\u7684\u5e8f\u5217\u4e0d\u5339\u914d\uff0c\u53ef\u4ee5\u4f7f\u8ad6\u6587\u7684\u7d50\u679c\u548c\u7d50\u8ad6\u7121\u6548\u3002\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u9451\u5225\u7d50\u679c\u53ef\u8aaa\u660e\uff1a\u8ad6\u6587\u4e2d\u7684\u7d50\u679c\u7121\u6cd5\u5c0d\u539f\u59cb\u5be6\u9a57\u4f5c\u771f\u5be6\u7684\u53cd\u6620\u3002\u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u7e3d\u5171\u572890\u591a\u7bc7\u8ad6\u6587\u4e2d\u767c\u73fe\u4e86\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u3002\u5f9e\u5982\u6b64\u7684\u932f\u8aa4\u770b\u4f86\uff0c\u6b63\u5728\u9032\u884c\u4e2d\u7684\u7814\u7a76\uff0c\u5176\u6709\u6548\u6027\u548c\u65b9\u6cd5\u5c0d\u65bc\u672a\u4f86\u7684\u5be6\u9a57\u53ef\u8907\u88fd\u6027(reproducibility)\u6709\u8457\u56b4\u91cd\u7684\u5f71\u97ff\u3002 \u5229\u7528\u300cSeek &amp; Blastn\u300d\u53ef\u4fc3\u9032\u826f\u597d\u7684\u79d1\u5b78\u5be6\u8e10\uff0c\u800c\u975e\u8d95\u76e1\u6bba\u7d55\u3002\u671f\u671b\u9019\u4e00\u985e\u5de5\u5177\u5c07\u53ef\u4f7f\u932f\u8aa4\u7387\u91cf\u5316\uff0c\u4ee5\u63a7\u7ba1\u9810\u9632\u8907\u88fd\u5be6\u9a57\u7684\u5371\u6a5f\u3002\u6b64\u5916\uff0cLabb\u00e9\u5148\u524d\u4f7f120\u7bc7\u8ad6\u6587\u906d\u64a4\u56de\uff0c\u9019\u4e5f\u662f\u4fc3\u6210\u51fa\u7248\u5546\u4f7f\u7528Seek &amp; Blastn\u5de5\u5177\u4f5c\u70ba\u6587\u7ae0\u7be9\u9078\u904e\u7a0b\u7684\u52d5\u529b\u3002\u57fa\u65bc\u8edf\u9ad4\u7684\u6210\u672c\u3001\u6e96\u78ba\u6027\u548c\u6613\u7528\u6027\uff0c\u5c07\u53ef\u671b\u63d0\u9ad8\u73fe\u6709\u7684\u5b78\u8853\u51fa\u7248\u6a19\u6e96\u3002\u5b78\u8853\u671f\u520a\u7684\u7de8\u8f2f\u53ef\u4ee5\u8207\u4e4b\u5171\u540c\u958b\u767c\u8edf\u9ad4\u7684\u8a66\u7528\u7248\u672c\uff0c\u5728\u7a69\u5b9a\u6210\u719f\u4e26\u4e14\u5efa\u7acb\u53ef\u4fe1\u5ea6\u4e4b\u5f8c\uff0c\u6709\u5229\u65bc\u96a8\u5f8c\u5c07\u9032\u884c\u7684\u5546\u696d\u5316\u3002<\/p>\n","protected":false},"author":2,"featured_media":3793,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_metadesc":"","footnotes":""},"categories":[159,161],"tags":[231],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v21.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Seek &amp; Blastn\uff1a\u5728\u764c\u75c7\u7814\u7a76\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u5e8f\u5217\u4e2d\u7684\u932f\u8aa4 - \u512a\u8b6f\u5802\u5b78\u82d1\uff0d\u7cbe\u8b6f\u6c42\u7cbe | Ulatus\u512a\u8b6f\u5802\u5c08\u696d\u5b78\u8853\u8ad6\u6587\u7ffb\u8b6f\u670d\u52d9\uff0c\u52a9\u60a8\u767b\u4e0a\u570b\u969b\u671f\u520a<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.enago.tw\/academy\/seek-\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Seek &amp; Blastn\uff1a\u5728\u764c\u75c7\u7814\u7a76\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u5e8f\u5217\u4e2d\u7684\u932f\u8aa4 - \u512a\u8b6f\u5802\u5b78\u82d1\uff0d\u7cbe\u8b6f\u6c42\u7cbe | Ulatus\u512a\u8b6f\u5802\u5c08\u696d\u5b78\u8853\u8ad6\u6587\u7ffb\u8b6f\u670d\u52d9\uff0c\u52a9\u60a8\u767b\u4e0a\u570b\u969b\u671f\u520a\" \/>\n<meta property=\"og:description\" content=\"2017\u5e7410\u6708\u521d\uff0c\u79d1\u5b78\u5bb6Jennifer Byrne\u548cCyril Labb\u00e9\u5206\u4eab\u4e86\u4e00\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u53ef\u4ee5\u5728\u5df2\u7d93\u767c\u8868\u7684\u7814\u7a76\u5be6\u9a57\u4e2d\uff0c\u6aa2\u6e2c\u51fa\u4e0d\u6b63\u78ba\u7684\u57fa\u56e0\u5e8f\u5217\u3002\u5229\u7528\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\uff0c\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u7684\u8ad6\u6587\u4e2d\uff0c\u767c\u73fe\u8d85\u904e\u4e8660\u7bc7\u6709\u5be6\u9a57\u7f3a\u9677\u3002\u9019\u5169\u4f4d\u79d1\u5b78\u5bb6\u662f\u5c07\u4ed6\u5011\u5728\u764c\u75c7\u7814\u7a76\u548c\u96fb\u8166\u7684\u5c08\u696d\u77e5\u8b58\u7d50\u5408\u8d77\u4f86\uff0c\u958b\u767c\u548c\u5c0e\u5165\u8edf\u9ad4\u300cSeek &amp; Blastn\u300d\u3002\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\u73fe\u5728\u6b63\u8655\u65bc\u6e2c\u8a66\u968e\u6bb5\uff0c\u53ef\u4f9b\u540c\u5115\u7814\u7a76\u4eba\u54e1\u4f5c\u7dda\u4e0a\u6e2c\u8a66\u548c\u6539\u9032\u3002\u4e0b\u4e00\u6b65\u5c07\u9032\u884c\u5546\u696d\u5316\uff0c\u4e26\u5411\u51fa\u7248\u5546\u548c\u671f\u520a\u7de8\u8f2f\u63a8\u85a6\u8a72\u8edf\u9ad4\u3002 \u81ea2015\u5e74\u4ee5\u4f86\uff0cByrne\u4fbf\u81f4\u529b\u65bc\u8b58\u5225\u4eba\u985e\u764c\u75c7\u8ad6\u6587\u4e2d\u7684\u932f\u8aa4\uff0c\u57285\u7bc7\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u529f\u80fd\u7684\u932f\u8aa4\u3002\u5728\u5be9\u67e5\u904e\u7a0b\u4e2d\uff0c\u767c\u73fe\u5831\u544a\u4e2d\u7684\u6838\u82f7\u9178\u5e8f\u5217(nucleotide sequences)\u662f\u4e0d\u6b63\u78ba\u7684\uff0c\u56e0\u6b64\u6709\u5169\u7bc7\u8ad6\u6587\u906d\u5230\u64a4\u56de\u3002\u5f8c\u7e8c\u9084\u6709\u5169\u7bc7\u8ad6\u6587\u572811\u670821\u65e5\u4ea6\u906d\u64a4\u56de\u3002\u9019\u4e9b\u932f\u8aa4\u53c8\u51fa\u73fe\u5728\u5176\u4ed625\u7bc7\u8ad6\u6587\u4e2d\uff0c\u4e5f\u4fc3\u6210Byrne\u548cLabb\u00e9\u6b63\u5f0f\u958b\u767c\u548c\u5c0e\u5165\u300cSeek &amp; Blastn\u300d\u6aa2\u6e2c\u5de5\u5177\u3002 \u5c0d\u65bc\u9019\u5957\u6aa2\u6e2c\u5de5\u5177\u7684\u539f\u7406\uff0c\u7c21\u800c\u8a00\u4e4b\uff0c\u5f9e\u4e0a\u50b3\u5230\u8edf\u9ad4\u7684\u4efb\u4f55\u7d66\u5b9a\u8ad6\u6587\u4e2d\u6240\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9032\u884c\u57fa\u65bc\u4e8b\u5be6\u7684\u6e2c\u8a66\u3002\u5f9e\u6280\u8853\u4f86\u8aaa\uff0c\u8edf\u9ad4\u7a0b\u5f0f\u4ea4\u53c9\u6bd4\u5c0d\u6aa2\u67e5\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9019\u662f\u7a31\u70ba\u6838\u82f7\u9178\u57fa\u672c\u5c40\u90e8\u8abf\u6574\u641c\u7d22\u5de5\u5177\uff08Nucleotide Basic Local Alignment Search tool\uff0cBLAST\uff09\u7684\u516c\u5171\u8cc7\u6599\u5eab\u3002\u5982\u679c\u63cf\u8ff0\u7684\u76ee\u6a19\u4eba\u985e\u57fa\u56e0\u5e8f\u5217\u8207Blastn\u8cc7\u6599\u5eab\u4e0d\u5339\u914d\uff0c\u5247\u6703\u6a19\u8a18\u932f\u8aa4\u3002\u76f8\u53cd\u7684\uff0c\u534a\u81ea\u52d5\u5316\u8edf\u9ad4\u7a0b\u5f0f\u4e5f\u53ef\u4ee5\u6aa2\u6e2c\u88ab\u63cf\u8ff0\u70ba\u975e\u76ee\u6a19\u7684\u5e8f\u5217\uff0c\u4e26\u4e14\u8207Blastn\u8cc7\u6599\u5eab\u5339\u914d\u3002\u5118\u7ba1\u76ee\u524d\u50c5\u9650\u65bc\u4eba\u985e\u5e8f\u5217\uff0c\u4f46\u662f\u5b83\u5011\u9084\u5e0c\u671b\u5305\u62ec\u5176\u4ed6\u7269\u7a2e\u7684\u5e8f\u5217\u9a57\u8b49\u3002 \u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u57289\u6708\u4efd\u5411\u570b\u969b\u540c\u5115\u5be9\u67e5\u548c\u79d1\u5b78\u51fa\u7248\u6703\u8b70\u63d0\u4ea4\u4e86\u4ed6\u5011\u8a08\u5283\u7684\u65e9\u671f\u7814\u7a76\u6210\u679c\u3002\u5728\u6703\u8b70\u8a18\u9304\u4e2d\u6982\u8ff0\u4e86\u8a72\u8a08\u5283\u5982\u4f55\u63d0\u9ad8\u79d1\u5b78\u54c1\u8cea\u548c\u4fe1\u8b7d\u3002\u6839\u64da\u8a2d\u8a08\uff0c\u300cSeek &amp; Blastn\u300d\u534a\u81ea\u52d5\u5316\u5de5\u5177\u4f7f\u7528\u5be6\u9ad4\u8b58\u5225\u6280\u8853\u63d0\u53d6\u4e86\u57fa\u56e0\u6a19\u8b58\u7b26\u865f\uff08gene identifier\uff09\u548c\u6838\u82f7\u9178\u5e8f\u5217\uff08nucleotide sequences\uff09\u3002\u4f7f\u7528\u6a5f\u5668\u81ea\u52d5\u5206\u6790\u5305\u542b\u6bcf\u500b\u5e8f\u5217\u7684\u57fa\u56e0\u53e5\uff0c\u8207blastn\u5206\u6790\u76f8\u6bd4\u8f03\uff0c\u9078\u5b9a\u4e00\u500b\u8072\u7a31\u72c0\u614b\u3002Google Scholar\u53ef\u4ee5\u9032\u4e00\u6b65\u8a55\u4f30\u6587\u737b\u4e2d\u7684\u8072\u7a31\u72c0\u614b\u3002\u9996\u5148\uff0c\u7814\u7a76\u9451\u5225\u51fa\u4e00\u7d44\u9ad8\u5ea6\u76f8\u4f3c\u7684\u764c\u75c7\u7814\u7a76\u51fa\u7248\u7269\uff08CorpusP\uff09\u3002\u9019\u9805\u7814\u7a76\u9032\u4e00\u6b65\u5305\u62ec\u4e86\u53e6\u5916\u4e00\u7d44154\u500b\u672a\u77e5\u7814\u7a76\uff08CorpusU\uff09\u3002 \u6839\u64da\u9019\u9805\u7814\u7a76\uff0c\u4f7f\u7528\u300cSeek &amp; Blastn\u300d\u5728\u9019\u4e9b\u51fa\u7248\u7269\u63d0\u53d6\u4e86\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u5176\u4e2dCorpusP\u670948\/48 (100%)\uff0c\u4ee5\u53caCorpusU\u6709111\/154(72%)\u3002\u5728CorpusP\u4e2d\u6a19\u8a18\u4e86\u4e0d\u6b63\u78ba\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u536038\/48 (79%)\u3002 \u6b64\u5916\uff0c\u8a72\u5de5\u5177\u8868\u660e\uff0c\u8207\u76ee\u6a19\u5e8f\u5217(targeting sequences)\u76f8\u6bd4\uff0c\u975e\u76ee\u6a19\u5e8f\u5217(nontargeting sequences)\u7684\u9810\u6e2c\u662f\u4e0d\u6b63\u78ba\u7684\u3002\u6aa2\u51fa\u6838\u82f7\u9178\u5e8f\u5217\u4e0d\u6b63\u78ba\u536030\/154\uff0819%\uff09\uff0c\u96d6\u7136\u7570\u5e38\u6bd4\u4f8b\u5f88\u5c0f\uff0c\u4f46\u4e8b\u5be6\u67e5\u6838\u7a0b\u5f0f\u4ecd\u7136\u5b58\u5728\u5be6\u8cea\u6027\u7684\u932f\u8aa4\u3002\u56e0\u6b64\uff0c\u5c07\u8ad6\u6587\u4e1f\u9032\u8edf\u9ad4\u67e5\u6838\u5f8c\uff0c\u76ee\u524d\u9084\u9700\u8981\u984d\u5916\u7684\u4eba\u5de5\u67e5\u6838\u3002\u7576\u57fa\u56e0\u8fa8\u8b58\u767c\u751f\u8b8a\u7570\u6642\uff0c\u7a0b\u5f0f\u4e5f\u6703\u5728\u76ee\u6a19\u5e8f\u5217\u5206\u6790\u4e2d\u4e5f\u6709\u98a8\u96aa\u3002\u76ee\u524dSeek &amp; Blastn\u53ea\u662f\u4e00\u500b\u5c0f\u898f\u6a21\u6e2c\u8a66\u6027\u8cea\u7684\u7a0b\u5f0f\uff0c\u4ecd\u9700\u8981\u751f\u91ab\u5b78\u754c\u7684\u540c\u5115\u5be9\u67e5\u4f5c\u5f8c\u7e8c\u7684\u5b8c\u6574\u5206\u6790\u3002 \u5118\u7ba1\u8a72\u8edf\u9ad4\u8655\u65bc\u521d\u6b65\u72c0\u614b\uff0c\u4f46\u5b83\u7a81\u986f\u51fa\u4e86\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u7684\u4e00\u4e9b\u6838\u5fc3\u554f\u984c\u3002\u4f8b\u5982\uff0c\u5728\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u9a57\u8b49\u51fa\u7684\u5e8f\u5217\u4e0d\u5339\u914d\uff0c\u53ef\u4ee5\u4f7f\u8ad6\u6587\u7684\u7d50\u679c\u548c\u7d50\u8ad6\u7121\u6548\u3002\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u9451\u5225\u7d50\u679c\u53ef\u8aaa\u660e\uff1a\u8ad6\u6587\u4e2d\u7684\u7d50\u679c\u7121\u6cd5\u5c0d\u539f\u59cb\u5be6\u9a57\u4f5c\u771f\u5be6\u7684\u53cd\u6620\u3002\u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u7e3d\u5171\u572890\u591a\u7bc7\u8ad6\u6587\u4e2d\u767c\u73fe\u4e86\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u3002\u5f9e\u5982\u6b64\u7684\u932f\u8aa4\u770b\u4f86\uff0c\u6b63\u5728\u9032\u884c\u4e2d\u7684\u7814\u7a76\uff0c\u5176\u6709\u6548\u6027\u548c\u65b9\u6cd5\u5c0d\u65bc\u672a\u4f86\u7684\u5be6\u9a57\u53ef\u8907\u88fd\u6027(reproducibility)\u6709\u8457\u56b4\u91cd\u7684\u5f71\u97ff\u3002 \u5229\u7528\u300cSeek &amp; Blastn\u300d\u53ef\u4fc3\u9032\u826f\u597d\u7684\u79d1\u5b78\u5be6\u8e10\uff0c\u800c\u975e\u8d95\u76e1\u6bba\u7d55\u3002\u671f\u671b\u9019\u4e00\u985e\u5de5\u5177\u5c07\u53ef\u4f7f\u932f\u8aa4\u7387\u91cf\u5316\uff0c\u4ee5\u63a7\u7ba1\u9810\u9632\u8907\u88fd\u5be6\u9a57\u7684\u5371\u6a5f\u3002\u6b64\u5916\uff0cLabb\u00e9\u5148\u524d\u4f7f120\u7bc7\u8ad6\u6587\u906d\u64a4\u56de\uff0c\u9019\u4e5f\u662f\u4fc3\u6210\u51fa\u7248\u5546\u4f7f\u7528Seek &amp; Blastn\u5de5\u5177\u4f5c\u70ba\u6587\u7ae0\u7be9\u9078\u904e\u7a0b\u7684\u52d5\u529b\u3002\u57fa\u65bc\u8edf\u9ad4\u7684\u6210\u672c\u3001\u6e96\u78ba\u6027\u548c\u6613\u7528\u6027\uff0c\u5c07\u53ef\u671b\u63d0\u9ad8\u73fe\u6709\u7684\u5b78\u8853\u51fa\u7248\u6a19\u6e96\u3002\u5b78\u8853\u671f\u520a\u7684\u7de8\u8f2f\u53ef\u4ee5\u8207\u4e4b\u5171\u540c\u958b\u767c\u8edf\u9ad4\u7684\u8a66\u7528\u7248\u672c\uff0c\u5728\u7a69\u5b9a\u6210\u719f\u4e26\u4e14\u5efa\u7acb\u53ef\u4fe1\u5ea6\u4e4b\u5f8c\uff0c\u6709\u5229\u65bc\u96a8\u5f8c\u5c07\u9032\u884c\u7684\u5546\u696d\u5316\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.enago.tw\/academy\/seek-\" \/>\n<meta property=\"og:site_name\" content=\"\u512a\u8b6f\u5802\u5b78\u82d1\uff0d\u7cbe\u8b6f\u6c42\u7cbe | Ulatus\u512a\u8b6f\u5802\u5c08\u696d\u5b78\u8853\u8ad6\u6587\u7ffb\u8b6f\u670d\u52d9\uff0c\u52a9\u60a8\u767b\u4e0a\u570b\u969b\u671f\u520a\" \/>\n<meta property=\"article:published_time\" content=\"2017-12-11T04:06:26+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-07-25T15:17:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.ulatus.tw\/academy\/wp-content\/uploads\/2017\/12\/ACACN-43.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"851\" \/>\n\t<meta property=\"og:image:height\" content=\"330\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Ulatus\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ulatus\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.ulatus.tw\/academy\/seek-blastn%ef%bc%9a%e5%9c%a8%e7%99%8c%e7%97%87%e7%a0%94%e7%a9%b6%e8%ab%96%e6%96%87%e4%b8%ad%e6%aa%a2%e6%b8%ac%e5%87%ba%e5%9f%ba%e5%9b%a0%e5%ba%8f%e5%88%97%e4%b8%ad%e7%9a%84%e9%8c%af%e8%aa%a4\/\",\"url\":\"https:\/\/www.enago.tw\/academy\/seek-\",\"name\":\"Seek & Blastn\uff1a\u5728\u764c\u75c7\u7814\u7a76\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u5e8f\u5217\u4e2d\u7684\u932f\u8aa4 - \u512a\u8b6f\u5802\u5b78\u82d1\uff0d\u7cbe\u8b6f\u6c42\u7cbe | Ulatus\u512a\u8b6f\u5802\u5c08\u696d\u5b78\u8853\u8ad6\u6587\u7ffb\u8b6f\u670d\u52d9\uff0c\u52a9\u60a8\u767b\u4e0a\u570b\u969b\u671f\u520a\",\"isPartOf\":{\"@id\":\"https:\/\/www.ulatus.tw\/academy\/#website\"},\"datePublished\":\"2017-12-11T04:06:26+00:00\",\"dateModified\":\"2020-07-25T15:17:29+00:00\",\"author\":{\"@id\":\"https:\/\/www.ulatus.tw\/academy\/#\/schema\/person\/e49a0b2f781090bd827d456a52e71803\"},\"breadcrumb\":{\"@id\":\"https:\/\/www.enago.tw\/academy\/seek-#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.enago.tw\/academy\/seek-\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.enago.tw\/academy\/seek-#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.ulatus.tw\/academy\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Seek &#038; 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Blastn\u300d\u3002\u9019\u500b\u8edf\u9ad4\u7a0b\u5f0f\u73fe\u5728\u6b63\u8655\u65bc\u6e2c\u8a66\u968e\u6bb5\uff0c\u53ef\u4f9b\u540c\u5115\u7814\u7a76\u4eba\u54e1\u4f5c\u7dda\u4e0a\u6e2c\u8a66\u548c\u6539\u9032\u3002\u4e0b\u4e00\u6b65\u5c07\u9032\u884c\u5546\u696d\u5316\uff0c\u4e26\u5411\u51fa\u7248\u5546\u548c\u671f\u520a\u7de8\u8f2f\u63a8\u85a6\u8a72\u8edf\u9ad4\u3002 \u81ea2015\u5e74\u4ee5\u4f86\uff0cByrne\u4fbf\u81f4\u529b\u65bc\u8b58\u5225\u4eba\u985e\u764c\u75c7\u8ad6\u6587\u4e2d\u7684\u932f\u8aa4\uff0c\u57285\u7bc7\u8ad6\u6587\u4e2d\u6aa2\u6e2c\u51fa\u57fa\u56e0\u529f\u80fd\u7684\u932f\u8aa4\u3002\u5728\u5be9\u67e5\u904e\u7a0b\u4e2d\uff0c\u767c\u73fe\u5831\u544a\u4e2d\u7684\u6838\u82f7\u9178\u5e8f\u5217(nucleotide sequences)\u662f\u4e0d\u6b63\u78ba\u7684\uff0c\u56e0\u6b64\u6709\u5169\u7bc7\u8ad6\u6587\u906d\u5230\u64a4\u56de\u3002\u5f8c\u7e8c\u9084\u6709\u5169\u7bc7\u8ad6\u6587\u572811\u670821\u65e5\u4ea6\u906d\u64a4\u56de\u3002\u9019\u4e9b\u932f\u8aa4\u53c8\u51fa\u73fe\u5728\u5176\u4ed625\u7bc7\u8ad6\u6587\u4e2d\uff0c\u4e5f\u4fc3\u6210Byrne\u548cLabb\u00e9\u6b63\u5f0f\u958b\u767c\u548c\u5c0e\u5165\u300cSeek &amp; Blastn\u300d\u6aa2\u6e2c\u5de5\u5177\u3002 \u5c0d\u65bc\u9019\u5957\u6aa2\u6e2c\u5de5\u5177\u7684\u539f\u7406\uff0c\u7c21\u800c\u8a00\u4e4b\uff0c\u5f9e\u4e0a\u50b3\u5230\u8edf\u9ad4\u7684\u4efb\u4f55\u7d66\u5b9a\u8ad6\u6587\u4e2d\u6240\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9032\u884c\u57fa\u65bc\u4e8b\u5be6\u7684\u6e2c\u8a66\u3002\u5f9e\u6280\u8853\u4f86\u8aaa\uff0c\u8edf\u9ad4\u7a0b\u5f0f\u4ea4\u53c9\u6bd4\u5c0d\u6aa2\u67e5\u9078\u51fa\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u9019\u662f\u7a31\u70ba\u6838\u82f7\u9178\u57fa\u672c\u5c40\u90e8\u8abf\u6574\u641c\u7d22\u5de5\u5177\uff08Nucleotide Basic Local Alignment Search tool\uff0cBLAST\uff09\u7684\u516c\u5171\u8cc7\u6599\u5eab\u3002\u5982\u679c\u63cf\u8ff0\u7684\u76ee\u6a19\u4eba\u985e\u57fa\u56e0\u5e8f\u5217\u8207Blastn\u8cc7\u6599\u5eab\u4e0d\u5339\u914d\uff0c\u5247\u6703\u6a19\u8a18\u932f\u8aa4\u3002\u76f8\u53cd\u7684\uff0c\u534a\u81ea\u52d5\u5316\u8edf\u9ad4\u7a0b\u5f0f\u4e5f\u53ef\u4ee5\u6aa2\u6e2c\u88ab\u63cf\u8ff0\u70ba\u975e\u76ee\u6a19\u7684\u5e8f\u5217\uff0c\u4e26\u4e14\u8207Blastn\u8cc7\u6599\u5eab\u5339\u914d\u3002\u5118\u7ba1\u76ee\u524d\u50c5\u9650\u65bc\u4eba\u985e\u5e8f\u5217\uff0c\u4f46\u662f\u5b83\u5011\u9084\u5e0c\u671b\u5305\u62ec\u5176\u4ed6\u7269\u7a2e\u7684\u5e8f\u5217\u9a57\u8b49\u3002 \u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u57289\u6708\u4efd\u5411\u570b\u969b\u540c\u5115\u5be9\u67e5\u548c\u79d1\u5b78\u51fa\u7248\u6703\u8b70\u63d0\u4ea4\u4e86\u4ed6\u5011\u8a08\u5283\u7684\u65e9\u671f\u7814\u7a76\u6210\u679c\u3002\u5728\u6703\u8b70\u8a18\u9304\u4e2d\u6982\u8ff0\u4e86\u8a72\u8a08\u5283\u5982\u4f55\u63d0\u9ad8\u79d1\u5b78\u54c1\u8cea\u548c\u4fe1\u8b7d\u3002\u6839\u64da\u8a2d\u8a08\uff0c\u300cSeek &amp; Blastn\u300d\u534a\u81ea\u52d5\u5316\u5de5\u5177\u4f7f\u7528\u5be6\u9ad4\u8b58\u5225\u6280\u8853\u63d0\u53d6\u4e86\u57fa\u56e0\u6a19\u8b58\u7b26\u865f\uff08gene identifier\uff09\u548c\u6838\u82f7\u9178\u5e8f\u5217\uff08nucleotide sequences\uff09\u3002\u4f7f\u7528\u6a5f\u5668\u81ea\u52d5\u5206\u6790\u5305\u542b\u6bcf\u500b\u5e8f\u5217\u7684\u57fa\u56e0\u53e5\uff0c\u8207blastn\u5206\u6790\u76f8\u6bd4\u8f03\uff0c\u9078\u5b9a\u4e00\u500b\u8072\u7a31\u72c0\u614b\u3002Google Scholar\u53ef\u4ee5\u9032\u4e00\u6b65\u8a55\u4f30\u6587\u737b\u4e2d\u7684\u8072\u7a31\u72c0\u614b\u3002\u9996\u5148\uff0c\u7814\u7a76\u9451\u5225\u51fa\u4e00\u7d44\u9ad8\u5ea6\u76f8\u4f3c\u7684\u764c\u75c7\u7814\u7a76\u51fa\u7248\u7269\uff08CorpusP\uff09\u3002\u9019\u9805\u7814\u7a76\u9032\u4e00\u6b65\u5305\u62ec\u4e86\u53e6\u5916\u4e00\u7d44154\u500b\u672a\u77e5\u7814\u7a76\uff08CorpusU\uff09\u3002 \u6839\u64da\u9019\u9805\u7814\u7a76\uff0c\u4f7f\u7528\u300cSeek &amp; Blastn\u300d\u5728\u9019\u4e9b\u51fa\u7248\u7269\u63d0\u53d6\u4e86\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u5176\u4e2dCorpusP\u670948\/48 (100%)\uff0c\u4ee5\u53caCorpusU\u6709111\/154(72%)\u3002\u5728CorpusP\u4e2d\u6a19\u8a18\u4e86\u4e0d\u6b63\u78ba\u7684\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u536038\/48 (79%)\u3002 \u6b64\u5916\uff0c\u8a72\u5de5\u5177\u8868\u660e\uff0c\u8207\u76ee\u6a19\u5e8f\u5217(targeting sequences)\u76f8\u6bd4\uff0c\u975e\u76ee\u6a19\u5e8f\u5217(nontargeting sequences)\u7684\u9810\u6e2c\u662f\u4e0d\u6b63\u78ba\u7684\u3002\u6aa2\u51fa\u6838\u82f7\u9178\u5e8f\u5217\u4e0d\u6b63\u78ba\u536030\/154\uff0819%\uff09\uff0c\u96d6\u7136\u7570\u5e38\u6bd4\u4f8b\u5f88\u5c0f\uff0c\u4f46\u4e8b\u5be6\u67e5\u6838\u7a0b\u5f0f\u4ecd\u7136\u5b58\u5728\u5be6\u8cea\u6027\u7684\u932f\u8aa4\u3002\u56e0\u6b64\uff0c\u5c07\u8ad6\u6587\u4e1f\u9032\u8edf\u9ad4\u67e5\u6838\u5f8c\uff0c\u76ee\u524d\u9084\u9700\u8981\u984d\u5916\u7684\u4eba\u5de5\u67e5\u6838\u3002\u7576\u57fa\u56e0\u8fa8\u8b58\u767c\u751f\u8b8a\u7570\u6642\uff0c\u7a0b\u5f0f\u4e5f\u6703\u5728\u76ee\u6a19\u5e8f\u5217\u5206\u6790\u4e2d\u4e5f\u6709\u98a8\u96aa\u3002\u76ee\u524dSeek &amp; Blastn\u53ea\u662f\u4e00\u500b\u5c0f\u898f\u6a21\u6e2c\u8a66\u6027\u8cea\u7684\u7a0b\u5f0f\uff0c\u4ecd\u9700\u8981\u751f\u91ab\u5b78\u754c\u7684\u540c\u5115\u5be9\u67e5\u4f5c\u5f8c\u7e8c\u7684\u5b8c\u6574\u5206\u6790\u3002 \u5118\u7ba1\u8a72\u8edf\u9ad4\u8655\u65bc\u521d\u6b65\u72c0\u614b\uff0c\u4f46\u5b83\u7a81\u986f\u51fa\u4e86\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u7684\u4e00\u4e9b\u6838\u5fc3\u554f\u984c\u3002\u4f8b\u5982\uff0c\u5728\u73fe\u6709\u51fa\u7248\u7269\u4e2d\u9a57\u8b49\u51fa\u7684\u5e8f\u5217\u4e0d\u5339\u914d\uff0c\u53ef\u4ee5\u4f7f\u8ad6\u6587\u7684\u7d50\u679c\u548c\u7d50\u8ad6\u7121\u6548\u3002\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u9451\u5225\u7d50\u679c\u53ef\u8aaa\u660e\uff1a\u8ad6\u6587\u4e2d\u7684\u7d50\u679c\u7121\u6cd5\u5c0d\u539f\u59cb\u5be6\u9a57\u4f5c\u771f\u5be6\u7684\u53cd\u6620\u3002\u9019\u5169\u4f4d\u7814\u7a76\u4eba\u54e1\u7e3d\u5171\u572890\u591a\u7bc7\u8ad6\u6587\u4e2d\u767c\u73fe\u4e86\u4e0d\u6b63\u78ba\u7684\u5e8f\u5217\u3002\u5f9e\u5982\u6b64\u7684\u932f\u8aa4\u770b\u4f86\uff0c\u6b63\u5728\u9032\u884c\u4e2d\u7684\u7814\u7a76\uff0c\u5176\u6709\u6548\u6027\u548c\u65b9\u6cd5\u5c0d\u65bc\u672a\u4f86\u7684\u5be6\u9a57\u53ef\u8907\u88fd\u6027(reproducibility)\u6709\u8457\u56b4\u91cd\u7684\u5f71\u97ff\u3002 \u5229\u7528\u300cSeek &amp; 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